Journal
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
Volume 9, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2021.770049
Keywords
large segment bone defect; GelMA; hydrogel; BMSCs; ECM
Funding
- National Key Research and Development Program of China [2018YFC1106800]
- National Natural Science Foundation of China [31971251, 81874002]
- Sichuan Province Science and Technology Department Projects [2016CZYD0004, 2019JDTD0008, 2019YFH0079, 2017SZ0195, 2019JDRC0100, 2020JDRC0054]
- 111 Project [B16033]
- China Postdoctoral Science Foundation [2021M692279]
- National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University [Y2018B22]
- West China Hospital Postdoctoral Research and Development Fund
- [Z20192013]
- [2019HXBH068]
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This study developed a photocrosslinked biomimetic methacrylated gelatin (Bio-GelMA) hydrogel scaffold to simulate the natural bone extracellular matrix, providing a 3D template and extracellular matrix microenvironment. Bone marrow mesenchymal stem cells (BMSCS) encapsulated in Bio-GelMA scaffolds showed therapeutic effects in a 3D environment for segmental bone defects, with good biocompatibility and mechanical properties. In vivo rat model experiments demonstrated that BMSC-loaded Bio-GelMA promoted robust new bone formation, indicating its potential for future clinical applications.
Large-segment bone defect caused by trauma or tumor is one of the most challenging problems in orthopedic clinics. Biomimetic materials for bone tissue engineering have developed dramatically in the past few decades. The organic combination of biomimetic materials and stem cells offers new strategies for tissue repair, and the fate of stem cells is closely related to their extracellular matrix (ECM) properties. In this study, a photocrosslinked biomimetic methacrylated gelatin (Bio-GelMA) hydrogel scaffold was prepared to simulate the physical structure and chemical composition of the natural bone extracellular matrix, providing a three-dimensional (3D) template and extracellular matrix microenvironment. Bone marrow mesenchymal stem cells (BMSCS) were encapsulated in Bio-GelMA scaffolds to examine the therapeutic effects of ECM-loaded cells in a 3D environment simulated for segmental bone defects. In vitro results showed that Bio-GelMA had good biocompatibility and sufficient mechanical properties (14.22kPa). A rat segmental bone defect model was constructed in vivo. The GelMA-BMSC suspension was added into the PDMS mold with the size of the bone defect and photocured as a scaffold. BMSC-loaded Bio-GelMA resulted in maximum and robust new bone formation compared with hydrogels alone and stem cell group. In conclusion, the bio-GelMA scaffold can be used as a cell carrier of BMSC to promote the repair of segmental bone defects and has great potential in future clinical applications.
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