Journal
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
Volume 9, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2021.782799
Keywords
parylene-C; micropillars; selenium nanoparticles; biocompatibility; antimicrobial
Funding
- GACR [GA19-04270Y]
- Czech Republic
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This study focused on the modification of Parylene-C surfaces with selenium nanoparticles as antibacterial agents and tested the designed surfaces against bacterial pathogens and mesenchymal stem cells. The results showed a bacteriostatic effect on E. coli but no antibacterial effect on S. aureus, as well as cytotoxic effects on cells at high nanoparticle deposition. These findings provide valuable information for potential antibacterial treatment of Parylene-C in biomedicine.
Parylene-based implants or coatings introduce surfaces suffering from bacteria colonization. Here, we synthesized polyvinylpyrrolidone-stabilized selenium nanoparticles (SeNPs) as the antibacterial agent, and various approaches are studied for their reproducible adsorption, and thus the modification of parylene-C-coated glass substrate. The nanoparticle deposition process is optimized in the nanoparticle concentration to obtain evenly distributed NPs on the flat parylene-C surface. Moreover, the array of parylene-C micropillars is fabricated by the plasma etching of parylene-C on a silicon wafer, and the surface is modified with SeNPs. All designed surfaces are tested against two bacterial pathogens, Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive). The results show no antibacterial effect toward S. aureus, while some bacteriostatic effect is observed for E. coli on the flat and microstructured parylene. However, SeNPs did not enhance the antibacterial effect against both bacteria. Additionally, all designed surfaces show cytotoxic effects toward mesenchymal stem cells at high SeNP deposition. These results provide valuable information about the potential antibacterial treatment of widely used parylene-C in biomedicine.
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