Journal
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
Volume 9, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fcell.2021.721795
Keywords
4-hydroxynonenal (4-HNE); myocardial ischemia; reperfusion injury (MIRI); necroptosis; RIP1 (RIPK1); ubiquitination
Categories
Funding
- National Natural Science Foundation of China [82030059, 82072141, 81873953]
- National S&T Fundamental Resources Investigation Project [2018FY100600, 2018FY100602]
- Taishan Pandeng Scholar Program of Shandong Province [tspd20181220]
- Key R&D Program of Shandong Province [2019GSF108261, 2018GSF118003, 2017GSF218040]
- Natural Science Foundation of Shandong Province [ZR2020MH030]
- Shandong Medical and Health Science Technology Development Plan Project [2018WS329]
- Clinical Research Foundation of Shandong University [2020SDUCRCC014]
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This study demonstrated that 4-HNE contributes to cardiomyocyte necroptosis by regulating RIP1 protein degradation, while ALDH2-Tg mice showed significantly reduced necroptosis. Perfusion of 4-HNE enhanced cardiomyocyte necroptosis in a time- and concentration-dependent manner, and knockdown of RIP1 prevented 4-HNE-induced necroptosis.
Background: Necroptosis is a vital regulator of myocardial ischemia/reperfusion (MI/R) injury. Meanwhile, 4-hydroxy-2-nonenal (4-HNE) is abundantly increased during MI/R injury. However, whether 4-HNE induces cardiomyocyte necroptosis during MI/R remains unknown. Methods: To observe the relationship between 4-HNE and necroptosis during MI/R, C57BL/6 mice and aldehyde dehydrogenase 2-transgenic (ALDH2-Tg) mice were both exposed to left anterior descending artery ligation surgery to establish MI/R injury models. For further study, isolated mouse hearts and H9c2 cells were both treated with 4-HNE to elucidate the underlying mechanisms. Results: Necroptosis and 4-HNE were both upregulated in I/R-injured hearts. Cardiomyocyte necroptosis was significantly decreased in I/R-injured hearts from ALDH2-Tg mice as compared with that of wild-type mice. In vitro studies showed that necroptosis was enhanced by 4-HNE perfusion in a time- and concentration-dependent manner. Knockdown of receptor-interacting serine/threonine-protein kinase 1 (RIP1) using small interfering RNA (siRNA) prevented 4-HNE-induced cardiomyocyte necroptosis, manifesting that RIP1 played a key role in the upregulation of cell necroptosis by 4-HNE. Further studies found that 4-HNE reduced the protein degradation of RIP1 by preventing K48-polyubiquitination of RIP1. Conclusion: 4-HNE contributes to cardiomyocyte necroptosis by regulating ubiquitin-mediated proteasome degradation of RIP1.
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