Journal
JOURNAL OF EXTRACELLULAR VESICLES
Volume 11, Issue 2, Pages -Publisher
WILEY
DOI: 10.1002/jev2.12197
Keywords
exosome; immunotherapy; lymphangiogenesis; pre-metastatic niche; sentinel lymph node
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Funding
- ETH Zurich
- Krebsliga Zurich
- Vontobel Foundation
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EV-mediated communication between melanoma and lymph node endothelial cells is identified as a new pathway involved in tumor progression and immune inhibition. These extracellular vesicles are transported rapidly to lymph nodes through lymphatic vessels and interact with endothelial cells and macrophages.
Tumour-draining lymph nodes (LNs) undergo massive remodelling including expansion of the lymphatic sinuses, a process that has been linked to lymphatic metastasis by creation of a pre-metastatic niche. However, the signals leading to these changes have not been completely understood. Here, we found that extracellular vesicles (EVs) derived from melanoma cells are rapidly transported by lymphatic vessels to draining LNs, where they selectively interact with lymphatic endothelial cells (LECs) as well as medullary sinus macrophages. Interestingly, uptake of melanoma EVs by LN-resident LECs was partly dependent on lymphatic VCAM-1 expression, and induced transcriptional changes as well as proliferation of those cells. Furthermore, melanoma EVs shuttled tumour antigens to LN LECs for cross-presentation on MHC-I, resulting in apoptosis induction in antigen-specific CD8(+) T cells. In conclusion, our data identify EV-mediated melanoma-LN LEC communication as a new pathway involved in tumour progression and tumour immune inhibition, suggesting that EV uptake or effector mechanisms in LECs might represent a new target for melanoma therapy.
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