Journal
RESEARCH
Volume 2022, Issue -, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.34133/2022/9783602
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Funding
- National Natural Science Foundation of China [21627807, 21904079, 21722506]
- China Postdoctoral Science Foundation [2018M631441]
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This study developed a novel approach to improve the efficiency and sensitivity of ultraviolet photodissociation tandem mass spectrometry (UVPD MS/MS) for structural determination of lipids by incorporating a chromophore to the C=C moiety in fatty acyls. The results demonstrated the effectiveness of this approach in identifying the C=C bond locations in unsaturated lipids from complex lipid extracts.
Unraveling the complexity of the lipidome requires the development of novel approaches to facilitate structural identification and characterization of lipid species with isomer-level discrimination. Ultraviolet photodissociation tandem mass spectrometry (UVPD MS/MS) is a promising tool for structure determination of lipids. The sensitivity of UVPD for lipid analysis however is limited mainly due to weak absorption of UV photons by a C=C. Herein, a C=C site-specific derivatization, the Paterno-Buchi (PB) reaction, was used to incorporate a chromophore to the C=C moiety in fatty acyls, leading to significantly improved UVPD efficiency and sensitivity for pinpointing C=C locations. The wavelength-dependent photodissociation of the PB products demonstrated 4-CF3-benzophenone as the best reagent for UVPD in terms of the efficiency of generating C=C diagnostic fragments and simplicity for C=C location assignments. We demonstrated the effectiveness of this approach for the shotgun profiling of C=C location isomers in different lipid classes from complex lipid extracts, highlighting its potential to advancing the identification of the C=C bond locations in unsaturated lipids.
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