Journal
SLAS DISCOVERY
Volume 27, Issue 2, Pages 95-106Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.slasd.2021.12.001
Keywords
Immuno-oncology; Cytometry; Phenotyping; Lymphocytes
Funding
- AstraZeneca Ltd.
Ask authors/readers for more resources
This study provides a comprehensive understanding of the SICR profile of T and NK cells in solid tumors, guiding the design and application of future IO molecules.
The field of Immuno-Oncology (JO) is evolving to utilise novel antibody backbones that can co-target multiple cell-surface stimulatory and inhibitory co-receptors (SICR). This approach necessitates a better understanding of SICR co-expression at the single-cell level on IO-relevant tumor-infiltrating leukocyte (TIL) cell types such as T and natural killer (NK) cells. Using high-dimensional flow cytometry we established a comprehensive SICR profile for tumor-resident T and NK cells across a range of human solid tumors where there is a clear need for improved immunotherapeutic intervention. Leveraging the power of our large flow panel, we performed deep-phenotyping of the critical CD8(+)CD39(+) Cytotoxic T Lymphocyte (CTL) population that is enriched for tumor-reactive cytotoxic cells, revealing subsets that are differentiated by their SICR profile, including three that are uniquely defined by NKG2A expression. This study establishes a comprehensive SICR phenotype for human TIL T and NK cells, providing insights to guide the design and application of the next generation of IO molecules.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available