Journal
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
Volume 67, Issue 3, Pages 288-297Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/09637486.2016.1153611
Keywords
sterol regulatory element binding protein-1; non-alcoholic fatty liver disease; medium-chain fatty acid; liver X receptor-alpha; Lipolysis
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Funding
- International Science and Technology Cooperation Program of China [2011DFA32770]
- International Science and Technology Cooperation Program of Jiangxi Province [20112BDH80004, 20123BDH80011]
- Science and Technology Program of Jiangxi Province [20143ACG70015]
- Research Program of State Key Laboratory of Food Science and Technology, Nanchang University [SKLF-KF-201009, SKLF-ZZA-201303, SKLF-ZZB-201517]
- Special Fund for Graduate Innovation
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Accumulation of lipids in the liver can lead to cell dysfunction and steatosis, an important factor in pathogenesis causing non-alcoholic fatty liver disease. The mechanisms related to lipid deposition in the liver, however, remain poorly understood. This study was aimed to investigate the effects of medium-chain fatty acid (MCFA) on the lipolysis and expression of lipid-sensing genes in human liver cells with steatosis. A cellular steatosis model, which is suitable to experimentally investigate the impact of fat accumulation in the liver, was established in human normal liver cells (LO2 cells) with a mixture of free fatty acids (oleate/palmitate, 2:1) at 200 mu m for 24 h incubation. MCFA was found to down-regulate expression of liver X receptor-alpha, sterol regulatory element binding protein-1, acetyl-CoA carboxylase, fatty acid synthase, CD 36 and lipoprotein lipase in this cellular model, and have positive effects on adipose triglyceride lipase and hormone-sensitive lipase. These results suggest that MCFA may reduce lipid accumulation by regulating key lipid-sensing genes in human liver cells with steatosis.
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