Journal
NATURE BIOMEDICAL ENGINEERING
Volume 5, Issue 11, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41551-021-00805-x
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Funding
- US National Institutes of Health [R01CA203737, R01CA206366, R01CA243023, R01CA222251]
- Indiana University Strategic Research Initiative fund
- Vera Bradley Foundation for Breast Cancer Research
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High-throughput screening in breast tumour organoids can identify epigenetic inhibitors that promote antigen presentation and enhance T-cell-mediated cytotoxicity.
In breast cancer, genetic heterogeneity, the lack of actionable targets and immune evasion all contribute to the limited clinical response rates to immune checkpoint blockade therapy. Here, we report a high-throughput screen based on the functional interaction of mouse- or patient-derived breast tumour organoids and tumour-specific cytotoxic T cells for the identification of epigenetic inhibitors that promote antigen presentation and potentiate T-cell-mediated cytotoxicity. We show that the epigenetic inhibitors GSK-LSD1, CUDC-101 and BML-210, identified by the screen, display antitumour activities in orthotopic mammary tumours in mice, that they upregulate antigen presentation mediated by the major histocompatibility complex class I on breast tumour cells and that treatment with BML-210 substantially sensitized breast tumours to the inhibitor of the checkpoint programmed death-1. Standardized measurements of tumour-cell killing activity facilitated by tumour-organoid-T-cell screens may help with the identification of candidate immunotherapeutics for a range of cancers. Epigenetic inhibitors that promote antigen presentation and potentiate T-cell-mediated cytotoxicity can be identified by a high-throughput screen of cytotoxic T-cell activity in breast tumour organoids.
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