Antimicrobial activity of eucalyptus essential oil against Pseudomonas invitro and potential application in refrigerated storage of pork meat

The objective of this study was to investigate the influence of eucalyptus essential oil (EO) on the Pseudomonas invitro and application in the pork meat. Eucalyptus oil showed the greatest antimicrobial activity to Pseudomonas among five EOs (tea tree oil, eucalyptus oil, clove oil, mentha oil and garlic oil) invitro. Eucalyptus oil treatment caused the increasing of membrane permeability and the modifications of ultrastructure and morphology of Pseudomonas spp. and P.aeruginosa. Treatment of refrigerated pork with eucalyptus oil led to a significant decrease P.spp. count, pH, total volatile basic nitrogen and 2-thiobarbituric acid values, as well as higher sensory scores of custom acceptance than those in control at the end of cold storage. It was conclude that eucalyptus EO is a potential alternative to chemical treatment to control the spoilage and extend the shelf life of refrigerated pork meat.