Serratiopeptidase: a statistical approach towards enhancement of fermentation and biomass product recovery

Serratiopeptidase is a proteolytic enzyme which finds implications as an inflammation reducing factor. The present study shows that the enzyme does not bind with LOX and not able to block lipoxygenase (LOX)-catalyzed SPMs (specialized pro-resolving mediators) biosynthesis, thereby offering no impediment in the natural resolution of inflammation, instead aiding in it. Prior to start the procedural input, the medium was selected and optimized using Plackett-Burman Design with 5 variables and 3 dummies for 12 runs. Based on Plackett-Bruman results, one variable at a time (OVAT) method was carried out for soy bean flour (defatted) and peptone which were studied to be the major components affecting the yield. Serratiopeptidase was produced at 5.0-6.0 g/L by shake flask level compared with 2.0-3.0 g/L with basal medium. In case of fermentation, adding specific feeding solutions helped in achieving maximum yield of more than 17 g/L. Study of feeding pattern of soy bean oil and ammonia solutions in order to keep a track of the growth phases, pH effects, and consequently the yield of Serratiopeptidase has well corroborated the consideration of optimized product yield, considering the conditions for the settling down of mass, filtration rate and temperature profile for drying of Serratiopeptidase.

Automated summary

The study shows that Serratiopeptidase enzyme does not hinder the natural resolution of inflammation, but assists in it. By optimizing the medium using Plackett-Burman Design, the production yield of Serratiopeptidase was increased, and specific feeding solutions during fermentation resulted in higher yields.