D-lactic acid production from hydrothermally pretreated, alkali delignified and enzymatically saccharified rockrose with the metabolic engineered Escherichia coli strain JU15

Rockrose lignocellulosic residues (RR) were selectively fractionated for hemicellulose separation using autohydrolysis, followed by an alkaline treatment to solubilize the lignin. The cellulose-enriched solids were used to study the effect of solid loading (SL: 2-10%) and enzyme dosage (ED: 6.34-23.66 FPU/g dry biomass) on saccharification using a Doehlert experimental design, followed by fermentation with the metabolic engineered Escherichia coli strain JU15 to produce D-lactic acid (DLA). Pretreatment increased glucan content and enzymatic digestibility up to 84%. A significant positive effect of SL and ED was found for glucose production, but SL negatively impacted glucose yield. DLA concentrations and productivity varied from 8.85 to 32.98 g/L and 1.11 to 2.17 g/(Lh), respectively. Overall process efficiency strongly depended on saccharification yield and varied from 33 to 71%. These results indicate that sequential autohydrolysis, delignification, and fermentation of RR may be a potential relevant strategy for D-lactic production in the biorefinery framework.

Automated summary

The study focused on sequential autohydrolysis, delignification, and fermentation of Rockrose lignocellulosic residues for D-lactic acid production. The results showed increased glucan content and enzymatic digestibility, with a significant positive effect of solid loading and enzyme dosage on glucose production.