Cloning and Functional Analysis of the Soybean GmRIQ2 Promoter

The RIQ gene can optimize the structure of light-harvesting complex II by regulating the degree of granum stacking, and ultimately participates in plant light stress responses. The GmRIQ2 gene contributes to plant photoprotection and may be involved in negative regulation of yield. To understand the regulatory mechanisms of GmRIQ2 expression, we isolated the GmRIQ2 promoter (PGmRIQ2) from the KenFeng 16 soybean genome. Sequence analysis showed that several cis-acting elements were involved in hormone responsiveness, seed-specific regulation, anaerobic induction and light responsiveness. PGmRIQ2 was expressed in soybean roots. GUS activity was detected in Arabidopsis thaliana rosette leaves, stems and roots, indicating that PGmRIQ2 tended to drive expression in rosette leaf vascular tissue and had spatiotemporal expression characteristics at the early growth stage. Three promoter deletion fragments of 450 bp, 284 bp and 194 bp were obtained by 5 ' deletion. These three fragments all drove GUS gene expression in Arabidopsis thaliana rosette leaves, stems and roots. GUS gene expression decreased with deletion fragment truncation. In addition, the PGmRIQ2 auxin (IAA), abscisic acid (ABA) and methyl jasmonate (MeJA) response elements were located at base pairs -1661~-450, -450~-284 and -284~-194, respectively, and the promoter was induced by IAA, ABA, MeJA and light.

Automated summary

The RIQ gene regulates the stacking of granum to optimize the structure of light-harvesting complex II and participates in plant light stress responses. The GmRIQ2 gene contributes to plant photoprotection and may negatively regulate yield. The GmRIQ2 promoter (PGmRIQ2) showed expression in soybean roots and had spatiotemporal expression characteristics in Arabidopsis thaliana rosette leaves, stems, and roots at the early growth stage. The promoter was induced by auxin, abscisic acid, methyl jasmonate, and light.