Genomic Predictions of Phenotypes and Pseudo-Phenotypes for Viral Nervous Necrosis Resistance, Cortisol Concentration, Antibody Titer and Body Weight in European Sea Bass

Simple Summary Selective breeding programs based on genomic data are still not a common practice in aquaculture, although genomic selection has been widely demonstrated to be advantageous when trait phenotyping is a difficult task. In this study, we investigated the accuracy of predicting the phenotype and the estimated breeding value (EBV) of three Bayesian models and a Random Forest algorithm exploiting the information of a genome-wide SNP panel for European sea bass. The genomic predictions were developed for mortality caused by viral nervous necrosis, post-stress cortisol concentration, antibody titer against nervous necrosis virus and body weight. Selective breeding based on genomic data is a possible option for improving these traits while overcoming difficulties related to individual phenotyping of the investigated traits. Our results evidenced that the EBV used as a pseudo-phenotype enhances the predictive performances of genomic models, and that EBV can be predicted with satisfactory accuracy. The genomic prediction of the EBV for mortality might also be used to classify the phenotype for the same trait. In European sea bass (Dicentrarchus labrax L.), the viral nervous necrosis mortality (MORT), post-stress cortisol concentration (HC), antibody titer (AT) against nervous necrosis virus and body weight (BW) show significant heritability, which makes selective breeding a possible option for their improvement. An experimental population (N = 650) generated by a commercial broodstock was phenotyped for the aforementioned traits and genotyped with a genome-wide SNP panel (16,075 markers). We compared the predictive accuracies of three Bayesian models (Bayes B, Bayes C and Bayesian Ridge Regression) and a machine-learning method (Random Forest). The prediction accuracy of the EBV for MORT was approximately 0.90, whereas the prediction accuracies of the EBV and the phenotype were 0.86 and 0.21 for HC, 0.79 and 0.26 for AT and 0.71 and 0.38 for BW. The genomic prediction of the EBV for MORT used to classify the phenotype for the same trait showed moderate classification performance. Genome-wide association studies confirmed the polygenic nature of MORT and demonstrated a complex genetic structure for HC and AT. Genomic predictions of the EBV for MORT could potentially be used to classify the phenotype of the same trait, though further investigations on a larger experimental population are needed.

Automated summary

Selective breeding programs based on genomic data are not common in aquaculture, but genomic selection has been proven advantageous in difficult trait phenotyping. This study investigated the accuracy of predicting phenotype and breeding value using genomic data for European sea bass. The results showed that using estimated breeding value as a pseudo-phenotype improved the predictive performance of genomic models, and that this value could be predicted accurately. Further investigations are needed to validate the potential use of genomic predictions for mortality classification in a larger population.