Epidemiology of Porcine Circovirus Type 2 Circulating in Wild Boars of Portugal during the 2018-2020 Hunting Seasons Suggests the Emergence of Genotype 2d

Simple Summary Porcine circovirus type 2 (PCV-2) is a swine disease associated with post-weaning multi-systemic wasting syndrome, which causes a high economic impact on animal production. Recently, new evidence has suggested the increasing circulation of the PCV-2d genotype. We screened wild boar stools collected from several districts across Portugal during the 2018-2020 hunting seasons, for PCV-2 and genetically characterized detected strains. From a total 76 stool samples of wild boar tested, two sequences were obtained, both of the PCV-2d genotype, showing the presence of this genotype in Portugal for the first time. Monitoring wild PCV-2 reservoirs is important for both veterinary public health and economic reasons. Porcine circovirus type 2 (PCV-2) is associated with several syndromes affecting swine, also known as porcine-circovirus-associated diseases, of which post-weaning multi-systemic wasting syndrome stands out due to its high economic impact on swine production. Recent data suggest the increasing circulation of the PCV-2d genotype in several countries worldwide. To provide updated data on PCV-2 genotypes currently circulating in swine in Portugal, we screened wild boar stools collected from several districts across Portugal, during the 2018-2020 hunting seasons, for PCV-2 and genetically characterized detected strains. From a total of 76 stool samples of wild boar tested by PCR for the partial PCV-2 ORF2 gene, two sequences were obtained (2/76; 2.6%, 95% confidence interval: 0.032-9.18). Bidirectional sequencing showed that the sequences were 100% identical and both of the PCV-2d genotype, showing for the first time the presence of this genotype in Portugal. Monitoring wild PCV-2 reservoirs is important for both veterinary public health and economic reasons, since PCV-2 infection has a strong economic impact on the swine industry.

Automated summary

This study investigated the presence of the PCV-2d genotype in wild boars in Portugal and emphasized the importance of monitoring wild PCV-2 reservoirs.