Journal
BIOMEDICAL JOURNAL
Volume 45, Issue 5, Pages 776-787Publisher
ELSEVIER
DOI: 10.1016/j.bj.2021.10.007
Keywords
Brain stem death; Transplantation; Endothelin-1; Inflammation; Cytokines; Troponin
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Inflammatory responses following brain stem death can affect the availability and quality of organs for transplantation, as well as cardiovascular physiology.
Introduction: Organs procured following brain stem death (BSD) are the main source of organ grafts for transplantation. However, BSD is associated with inflammatory responses that may damage the organ and affect both the quantity and quality of organs available for transplant. Therefore, we aimed to investigate plasma andbronchoalveolar lavage (BAL) pro-inflammatory cytokine profiles and cardiovascular physiology in a clinically relevant 6-h ovinemodel of BSD. Methods: Twelve healthy female sheep (37-42 Kg) were anaesthetized and mechanically ventilated prior to undergoing BSD induction and then monitored for 6 h. Plasma and BAL endothelin-1 and cytokines (IL-1b, 6, 8 and tumour necrosis factor alpha (TNF-a)) were assessed by ELISA. Differential white blood cell counts were performed. Cardiac function during BSD was also examined using echocardiography, and cardiac biomarkers (A-type natriuretic peptide and troponin I were measured in plasma. Results: Plasma concentrations big ET-1, IL-6, IL-8, TNF-a and BAL IL-8 were significantly (p < 0.01) increased over baseline at 6 h post-BSD. Increased numbers of neutrophils were observed in the whole blood (3.1 x 10(9) cells/L [95% confidence interval (CI) 2.06-4.14] vs. 6 x 10(9) cells/L [95%CI 3.92-7.97]; p< 0.01) andBAL (4.5 x 10(9) cells/L [95%CI0.41-9.41] vs. 26[95%CI 12.29e39.80]; p = 0.03) after 6 h of BSD induction vs baseline. A significant increase in ANP production(20.28pM[95%CI 16.18-24.37] vs. 78.68pM[95%CI 53.16-104.21]; p< 0.0001) and cTnI release (0.039 ng/mL vs. 4.26 [95%CI 2.69-5.83] ng/mL; p < 0.0001), associated with a significant reduction in heart contractile function, were observed between baseline and 6 h. Conclusions: BSD induced systemic pro-inflammatory responses, characterized by increased neutrophil infiltration and cytokine production in the circulation and BAL fluid, and associated with reduced heart contractile function in ovine model of BSD.
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