Wolbachia Detection in Field-Collected Mosquitoes from Cameroon

Simple Summary Wolbachia bacteria from different strains, carried by many insects and nematodes, can interact in many ways with their hosts by changing their biology in different ways, including by suppressing vector population and reducing parasite transmission. Consequently, Wolbachia play an important role in vector control strategies. This study assessed the prevalence of natural Wolbachia infections in mosquitoes collected in Cameroon. Despite the low prevalence that was revealed, Wolbachia spp. were found in eight species of field-collected mosquitoes and are closely related to clades A and B. Aedes aegypti and A. gambiae sl., the main vectors of dengue and malaria, respectively, were not infected in this study, while C. moucheti recorded a high prevalence (46.67%). Future characterisation of the Wolbachia bacteria obtained is needed. Wolbachia spp., known to be maternally inherited intracellular bacteria, are widespread among arthropods, including mosquitoes. Our study assessed the presence and prevalence of Wolbachia infection in wild mosquitoes collected in Cameroon, using the combination of 23s rRNA Anaplasmatacea and 16s rRNA Wolbachia genes. Mosquitoes that were positive for Wolbachia were sequenced for subsequent phylogenetic analysis. Out of a total of 1740 individual mosquitoes belonging to 22 species and five genera screened, 33 mosquitoes (1.87%) belonging to eight species (namely, Aedes albopictus, A. contigus, Culex quinquefasciatus, C. perfuscus, C. wigglesworthi, C. duttoni, Anopheles paludis and Coquillettidia sp.) were found to be positive for Wolbachia infections. Wolbachia spp. were absent in A. gambiae and A. aegypti, the main vectors of malaria and dengue, respectively. Phylogenetic analysis of the 16S RNA sequences showed they belong mainly to two distinct subgroups (A and B). This study reports the presence of Wolbachia in about eight species of mosquitoes in Cameroon and suggests that future characterisation of the strains is needed.

Automated summary

Wolbachia bacteria, which can change host biology and suppress vector population, were found in 8 out of 22 mosquito species collected in Cameroon. Main vectors of diseases like dengue and malaria were not infected, while other species like C. moucheti showed relatively high infection rates. Further characterization of the Wolbachia strains is warranted.