Journal
NPJ VACCINES
Volume 6, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41541-021-00395-4
Keywords
-
Categories
Funding
- National Health and Medical Research Development grant from the Australian Government [AP1156063]
- National Health and Medical Research Development grant from the Coalition for Epidemic Preparedness Innovations (CEPI)
- Australian Postgraduate Award
Ask authors/readers for more resources
Influenza viruses cause a significant number of infections and deaths annually. Development of a universal influenza vaccine is challenging, but a modular trimerization domain known as the molecular clamp shows promise for rapid response vaccine development. The clamp-stabilised HA proteins demonstrate cross protection and serve as a proof-of-concept for future pandemic response vaccines.
Influenza viruses cause a significant number of infections and deaths annually. In addition to seasonal infections, the risk of an influenza virus pandemic emerging is extremely high owing to the large reservoir of diverse influenza viruses found in animals and the co-circulation of many influenza subtypes which can reassort into novel strains. Development of a universal influenza vaccine has proven extremely challenging. In the absence of such a vaccine, rapid response technologies provide the best potential to counter a novel influenza outbreak. Here, we demonstrate that a modular trimerization domain known as the molecular clamp allows the efficient production and purification of conformationally stabilised prefusion hemagglutinin (HA) from a diverse range of influenza A subtypes. These clamp-stabilised HA proteins provided robust protection from homologous virus challenge in mouse and ferret models and some cross protection against heterologous virus challenge. This work provides a proof-of-concept for clamp-stabilised HA vaccines as a tool for rapid response vaccine development against future influenza A virus pandemics.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available