Journal
PHARMACEUTICS
Volume 13, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/pharmaceutics13122152
Keywords
electrospun wound dressing; multifunctional structures; wound healing; chitosan; gelatin; phlorotannin-enriched extract; chronic wounds
Categories
Funding
- Fundacao para a Ciencia e a Tecnologia (FCT) [UIDB/04044/2020, UIDP/04044/2020]
- MATIS [CENTRO-01-0145-FEDER-000014-3362]
- SpinningTNT [POCI-01-02B7-FEDER-069285]
- European Union through EASME Blue Labs project AMALIA, Algae-to-MArket Lab IdeAs [EASME/EMFF/2016/1.2.1.4/03/SI2.750419]
- European Union through project VALORMAR (Mobilizing R&TD Programs, Portugal 2020) [POCI-01-0247-FEDER-024517]
- Integrated Programme of SR&TD Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate [Centro-01-0145-FEDER-000018]
- ORCHESTRA - FEDER-Fundo Europeu de Desenvolvimento Regional da Uniao Europeia, Portugal 2020, through COMPETE 2020-Programa Operacional Competitividade e Internacionalizacao [POCI-01-0247-FEDER-070155]
- FCT
- [PAMI-ROTEIRO/0328/2013]
- [022158]
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The study developed a multifunctional wound dressing using a gelatin/chitosan hybrid fibers and phlorotannin-enriched extract from the seaweed Undaria pinnatifida. The incorporation of the extract enhanced the elastic performance and stability of the electrospun structure, with prolonged release profile reaching up to 160 days. The antimicrobial activity against Pseudomonas aeruginosa and Staphylococcus aureus and the lack of cytotoxicity effects on hDNF cells make the electrospun structure a promising bioactive structure for potential use as a drug delivery system for skin regeneration.
The similarities of electrospun fibers with the skin extracellular matrix (ECM) make them promising structures for advanced wound dressings. Moreover, infection and resistance in wounds are a major health concern that may be reduced with antibacterial wound dressings. In this work, a multifunctional wound dressing was developed based on gelatin/chitosan hybrid fibers dopped with phlorotannin-enrich extract from the seaweed Undaria pinnatifida. The intrinsic electrospun structure properties combined with the antimicrobial and anti-inflammatory properties of phlorotannin-enrich extract will enhance the wound healing process. Electrospun meshes were produced by incorporating 1 or 2 wt% of extract, and the structure without extract was used as a control. Physico-chemical, mechanical, and biological properties were evaluated for all conditions. Results demonstrated that all developed samples presented a homogenous fiber deposition with the average diameters closer to the native ECM fibrils, and high porosities (~90%) that will be crucial to control the wound moist environment. According to the tensile test assays, the incorporation of phlorotannin-enriched extract enhances the elastic performance of the samples. Additionally, the extract incorporation made the structure stable over time since its in vitro degradation rates decreased under enzymatic medium. Extract release profile demonstrated a longstanding delivery (up to 160 days), reaching a maximum value of ~98% over time. Moreover, the preliminary antimicrobial results confirm the mesh's antimicrobial activity against Pseudomonas aeruginosa and Staphylococcus aureus. In terms of biological characterization, no condition presented cytotoxicity effects on hDNF cells, allowing their adhesion and proliferation over 14 days, except the condition of 2 wt% after 7 days. Overall, the electrospun structure comprising phlorotannins-enriched extract is a promising bioactive structure with potential to be used as a drug delivery system for skin regeneration by reducing the bacterial infection in the wound bed.
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