Biofilms can act as plasmid reserves in the absence of plasmid specific selection

Plasmids facilitate rapid bacterial adaptation by shuttling a wide variety of beneficial traits across microbial communities. However, under non-selective conditions, maintaining a plasmid can be costly to the host cell. Nonetheless, plasmids are ubiquitous in nature where bacteria adopt their dominant mode of life - biofilms. Here, we demonstrate that biofilms can act as spatiotemporal reserves for plasmids, allowing them to persist even under non-selective conditions. However, under these conditions, spatial stratification of plasmid-carrying cells may promote the dispersal of cells without plasmids, and biofilms may thus act as plasmid sinks.

Automated summary

Plasmids can be stored in biofilms, allowing them to persist even under non-selective conditions. However, under these conditions, cells carrying plasmids may promote the dispersal of cells without plasmids, turning biofilms into plasmid sinks.