Journal
ADVANCED MATERIALS TECHNOLOGIES
Volume 7, Issue 5, Pages -Publisher
WILEY
DOI: 10.1002/admt.202101141
Keywords
binding kinetics; multivalency; switchSENSE technology; virus-peptide interaction
Categories
Funding
- Bundesministerium fur Bildung und Forschung as part of the Bio-Chance-Plus/KMU-Innovativ fund
Ask authors/readers for more resources
Electrically controllable DNA nanolevers were used to study the binding interaction between Influenza A virus and PeB peptide. The study showed that the virus specifically binds to immobilized peptides, and can be used to characterize rate constants of virus-receptor interactions.
Electrically controllable deoxyribonuclic acid (DNA) nanolevers are used to investigate the binding interaction between Influenza A/Aichi/2/1968 and the peptide called PeB, which specifically binds the viral surface protein hemagglutinin. PeB is immobilized on gold electrodes of a switchSENSE biochip by conjugation to DNA-strands that are hybridized to complementary anchors. The surface-tethered DNA strand carries a fluorophore while the complementary strand is a multivalent arrangement carrying up to three PeB peptides. The nanolevers are kept upright (static) by applying a negative potential. Signal read-out for this static measurement mode is the change in fluorescence intensity due to changes in the local environment of the dye upon binding. Measurements of virus-peptide interaction show that the virus material specifically binds to the immobilized peptides and remains bound throughout the measurement time. Immobilized viruses are subsequently used as ligands to characterize oligovalent peptide binding to hemagglutinin, revealing rate constants of the interaction. Moreover, three Influenza A subtypes are compared in their binding behavior. Overall, this paper shows the ability to immobilize virus material on a sensor surface, which allows to target virus-proteins in their native environment. The switchSENSE method is therefore applicable to characterize virus-receptor interactions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available