Journal
ADVANCED MATERIALS TECHNOLOGIES
Volume 7, Issue 6, Pages -Publisher
WILEY
DOI: 10.1002/admt.202101121
Keywords
additive manufacturing; lab on chip; nucleic acid; electroanalysis
Categories
Funding
- project Advanced Functional Nanorobots - EFRR [CZ.02.1.01/0.0/0.0/15_003/0000444]
- Ministry of Education, Youth and Sports (Czech Republic) under ERC CZ program [LL2002]
- Ministry of Health of the Czech Republic [NU21-08-00407]
- Jose Castillejo program (Spain)
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Additive manufacturing technology, also known as 3D printing technology, has broad applications from nanosensors fabrication to 3D printing of buildings. Combining with real-time screening, the use of 3D printing for fast sensor development is significant during the COVID-19 pandemic.
Additive manufacturing technology, referred as 3D printing technology, is a growing research field with broad applications from nanosensors fabrication to 3D printing of buildings. Nowadays, the world is dealing with a pandemic and requires the use of simple sensing systems. Here, the strengths of fast screening by a lab-on-a-chip device through electrochemical detection using 3D printing technology for SARS-CoV-2 sensing are combined. This system comprises a PDMS microfluidic channel integrated with an electrochemical cell fully 3D-printed by a 3D printing pen (3D-PP). The 3D-PP genosensor is modified with an ssDNA probe that targeted the N gene sequence of SARS-CoV-2. The sensing mechanism relies on the electro-oxidation of adenines present in ssDNA when in contact with SARS-CoV-2 RNA. The hybridization between ssDNA and target RNA takes a place and ssDNA is desorbed from the genosensor surface, causing a decrease of the sensor signal. The developed SARS-CoV-2/3D-PP genosensor shows high sensitivity and fast response.
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