Changes in Epigenetic Patterns Related to DNA Replication in Vicia faba Root Meristem Cells under Cadmium-Induced Stress Conditions

Experiments on Vicia faba root meristem cells exposed to 150 mu M cadmium chloride (CdCl2) were undertaken to analyse epigenetic changes, mainly with respect to DNA replication stress. Histone modifications examined by means of immunofluorescence labeling included: (1) acetylation of histone H3 on lysine 56 (H3K56Ac), involved in transcription, S phase, and response to DNA damage during DNA biosynthesis; (2) dimethylation of histone H3 on lysine 79 (H3K79Me2), correlated with the replication initiation; (3) phosphorylation of histone H3 on threonine 45 (H3T45Ph), engaged in DNA synthesis and apoptosis. Moreover, immunostaining using specific antibodies against 5-MetC-modified DNA was used to determine the level of DNA methylation. A significant decrease in the level of H3K79Me2, noted in all phases of the CdCl2-treated interphase cell nuclei, was found to correspond with: (1) an increase in the mean number of intranuclear foci of H3K56Ac histones (observed mainly in S-phase), (2) a plethora of nuclear and nucleolar labeling patterns (combined with a general decrease in H3T45Ph), and (3) a decrease in DNA methylation. All these changes correlate well with a general viewpoint that DNA modifications and post-translational histone modifications play an important role in gene expression and plant development under cadmium-induced stress conditions.

Automated summary

The experiments on Vicia faba root meristem cells exposed to 150 mu M cadmium chloride analyzed the epigenetic changes, specifically focusing on DNA replication stress. The findings revealed a significant decrease in H3K79Me2 levels in CdCl2-treated interphase cell nuclei, along with an increase in H3K56Ac histone foci, a variety of nuclear and nucleolar labeling patterns, and a decrease in DNA methylation. These changes indicate the important role of DNA and histone modifications in gene expression and plant development under cadmium-induced stress conditions.