Journal
CELLS
Volume 10, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/cells10102737
Keywords
platelet distribution width; P-selectin; coagulation; VWF; mean platelet volume; thrombo-inflammation
Categories
Funding
- Telethon foundation [GGP04198]
- Italian Ministry of University and Research (MIUR) [1588-19/11/2004]
- European Union [798841]
- Italian Ministry of Health [GR-2018-12366528]
- Fondazione Umberto Veronesi
- Marie Curie Actions (MSCA) [798841] Funding Source: Marie Curie Actions (MSCA)
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Platelet distribution width (PDW) can serve as a simple and useful marker for platelet activation, specifically P-selectin dependent, with no sex differences, while mean platelet volume (MPV) shows no significant association. Further studies in larger cohorts are needed to fully understand the predictive value of PDW in thrombo-inflammatory conditions.
Defined as an index of platelet size heterogeneity, the platelet distribution width (PDW) is still a poorly characterized marker of platelet function in (sub)clinical disease. We presently validated PDW as a marker of P-selectin dependent platelet activation in the Moli-family cohort. Platelet-bound P-selectin and platelet/leukocyte mixed aggregates were measured by flow cytometry in freshly collected venous blood, both before and after in vitro platelet activation, and coagulation time was assessed in unstimulated and LPS- or TNF alpha-stimulated whole blood. Closure Times (CT) were measured in a Platelet Function Analyzer (PFA)-100. Multivariable linear mixed effect regression models (with age, sex and platelet count as fixed and family structure as random effect) revealed PDW to be negatively associated with platelet P-selectin, platelet/leukocyte aggregates and von Willebrand factor (VWF), and positively with PFA-100 CT, and LPS- and TNF-alpha-stimulated coagulation times. With the exception of VWF, all relationships were sex-independent. In contrast, no association was found between mean platelet volume (MPV) and these variables. PDW seems a simple, useful marker of ex vivo and in vitro P-selectin dependent platelet activation. Investigations of larger cohorts will define the usefulness of PDW as a risk predictor of thrombo-inflammatory conditions where activated platelets play a contributing role.
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