Journal
INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH
Volume 27, Issue 1, Pages 1-10Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09603123.2016.1246653
Keywords
Water quality; real-time quantitative PCR; Saprolegnia parasitica; river water; drinking water
Funding
- Zone Atelier de l'Arc Jurassien (ZAAJ)
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Since 2010, the Loue River (Franche-Comte, East of France) has been suffering from massive fish kills infested by Saprolegnia parasitica. The river supplies inhabitants of the city of Besancon in drinking water, raising the question of a potential risk through both water consumption and use. We developed a real-time quantitative PCR (qPCR) to quantify S. parasitica in the Loue River as well as in the drinking water. A weak spatial trend is suggested with greater quantities of S. parasitica observed at the sampling station close to the main pumping station. No S. parasitica DNA was detected in the tap water connected to pumping stations. The use of qPCR, which combines specificity, practicality, speed and reliability, appears to be an effective tool to monitor the spatial and temporal dynamics of this oomycete and identify the risk period for wild salmonid populations in the field, for fishery management or in aquaculture.
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