4.3 Article

Quantification of Saprolegnia parasitica in river water using real-time quantitative PCR: from massive fish mortality to tap drinking water

Journal

Publisher

TAYLOR & FRANCIS LTD
DOI: 10.1080/09603123.2016.1246653

Keywords

Water quality; real-time quantitative PCR; Saprolegnia parasitica; river water; drinking water

Funding

  1. Zone Atelier de l'Arc Jurassien (ZAAJ)

Ask authors/readers for more resources

Since 2010, the Loue River (Franche-Comte, East of France) has been suffering from massive fish kills infested by Saprolegnia parasitica. The river supplies inhabitants of the city of Besancon in drinking water, raising the question of a potential risk through both water consumption and use. We developed a real-time quantitative PCR (qPCR) to quantify S. parasitica in the Loue River as well as in the drinking water. A weak spatial trend is suggested with greater quantities of S. parasitica observed at the sampling station close to the main pumping station. No S. parasitica DNA was detected in the tap water connected to pumping stations. The use of qPCR, which combines specificity, practicality, speed and reliability, appears to be an effective tool to monitor the spatial and temporal dynamics of this oomycete and identify the risk period for wild salmonid populations in the field, for fishery management or in aquaculture.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.3
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available