Journal
CANCERS
Volume 13, Issue 22, Pages -Publisher
MDPI
DOI: 10.3390/cancers13225667
Keywords
cannabidiol; HIF-1 alpha; angiogenesis; stemness; breast cancer
Categories
Funding
- National Research Foundation of Korea [NRF-2018R1C1B5046445]
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CBD has been shown to suppress angiogenesis and stem cell-like properties of breast cancer by interfering with the Src/VHL/HIF-1a signaling pathway. This suggests that CBD may have potential for the treatment of refractory or recurrent breast cancer.
Cannabidiol (CBD), one of the compounds present in the marijuana plant, has antitumor properties. However, the effect of CBD on breast cancer remains unclear. The aim of this study was to assess the effects of CBD for the angiogenesis and stemness of breast cancer cells by decreasing the expression of hypoxia-induced factor-1a (HIF-1a) through the Src/von Hippel-Lindau tumor suppressor protein (VHL) interaction. CBD can suppress angiogenesis and stem cell-like properties of breast cancer through Src/VHL/HIF-1a signaling. To assess the effect of Cannabidiol (CBD) on the angiogenesis and stemness of breast cancer cells as well as proliferation. Methods: mRNA level and the amount of protein of vascular endothelial growth factor (VEGF) were determined by qRT-PCR and ELISA. The angiogenic potential of breast cancer cells under hypoxic conditions was identified by the HUVEC tube formation assay. The degradation of HIF-1a by CBD and the Src/von Hippel-Lindau tumor suppressor protein (VHL) interaction were assessed by a co-immunoprecipitation assay and Western blotting. To identify the stemness of mamospheres, they were evaluated by the sphere-forming assay and flow cytometry. Results: CBD can suppress angiogenesis and stem cell-like properties of breast cancer through Src/VHL/HIF-1a signaling. CBD may potentially be utilized in the treatment of refractory or recurrent breast cancer.
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