Anti-Tumor Activity of Expanded PBMC-Derived NK Cells by Feeder-Free Protocol in Ovarian Cancer

Simple Summary:& nbsp;Natural killer (NK) cell-based cancer therapies have substantially reshaped modern strategies for clinical cancer treatment and have improved outcomes for countless patients, notably in hematologic malignancies. Unfortunately, its efficacy is limited against solid tumors due to poor infiltration and persistence in the tumor microenvironment. In this study, we show that peripheral blood mononuclear cell-derived NK cell cytotoxicity and persistence can be greatly enhanced to provide highly potent and durable anti-tumor activity through expansion in a feeder cell free-expansion system. Importantly, we show expanded-NK efficacy against three distinct forms of ovarian cancer in mouse models (i.e., solid tumors, abdominal metastatic tumors, and ascites). Natural killer (NK) cells have shown great therapeutic potential against a wide range of cancers due to their pan-specific target recognition. Numerous reports indicate that NK cell immunotherapy is an effective therapeutic approach for treating hematological malignancies, but shows limited effects against solid tumors. In this study, several models of ovarian cancer (OC) were used to test the anti-cancer effects of NK cells derived from human peripheral blood mononuclear cells and expanded using a feeder cell-free expansion system (eNKs). The results show that eNKs exhibit potent inhibitory activity on tumor growth in different ovarian cancer xenograft mice (i.e., solid tumors, abdominal metastatic tumors, and ascites), importantly, in a dose-dependent manner. Moreover, adoptive transfer of eNKs resulted in significant reduction in ascites formation in OC peritoneal tumor models, and especially in reducing intraperitoneal ascites. We found that eNKs could migrate to the tumor site, retain their activity, and proliferate to maintain high cell counts in cutaneous xenograft mice. In addition, when increased the infusion with a high dose of 12 x 10(7) cells/mouse, Graft-versus-host disease could be induced by eNK. These data show that eNK cell immunotherapy could be a promising treatment strategy for ovarian cancers, including solid tumors and ascites.

Automated summary

NK cells have shown great potential in targeting various cancers, especially hematological malignancies, but their efficacy against solid tumors is limited. This study demonstrates that NK cells expanded using a feeder cell-free system derived from peripheral blood mononuclear cells have potent inhibitory effects on ovarian cancer growth. Adoptive transfer of expanded NK cells can reduce ascites formation and inhibit tumor growth in ovarian cancer mouse models. This highlights the promise of NK cell immunotherapy as a treatment strategy for ovarian cancers, including solid tumors and ascites.