4.6 Article

High diagnostic performance of independent alpha-synuclein seed amplification assays for detection of early Parkinson's disease

Related references

Note: Only part of the references are listed.
Article Clinical Neurology

Skin α-Synuclein Aggregation Seeding Activity as a Novel Biomarker for Parkinson Disease

Zerui Wang et al.

Summary: The study demonstrates that skin alpha Syn(P) seeding activity can potentially serve as a novel biomarker for antemortem diagnosis of Parkinson disease (PD) and other synucleinopathies. The analysis of skin samples from autopsies and biopsies shows a significant increase in alpha Syn(P) seeding activity in individuals with PD and synucleinopathies compared to controls with tauopathies and non-neurodegenerative diseases.

JAMA NEUROLOGY (2021)

Article Clinical Neurology

A rapid α-synuclein seed assay of Parkinson's disease CSF panel shows high diagnostic accuracy

Christina D. Orru et al.

Summary: The study shows that an improved assay for diagnosing Parkinson's disease dramatically reduces the diagnosis time while maintaining high performance standards. Positive signals were found in 97% of Parkinson's disease cases compared to only 13% of healthy controls, with weak correlations between assay parameters and clinical measures observed. The assay's efficiency in diagnosing Parkinson's disease is supported by these findings.

ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY (2021)

Article Clinical Neurology

Heterogeneity in α-synuclein fibril activity correlates to disease phenotypes in Lewy body dementia

Arpine Sokratian et al.

Summary: This study developed a new method to measure alpha-synuclein fibril-templating activity and found heterogeneity in fibril activity among patients with Lewy body dementia. Results indicated that quantitatively assessing fibril activities in cerebrospinal fluid can help stratify patient populations and measure therapeutic responses.

ACTA NEUROPATHOLOGICA (2021)

Article Clinical Neurology

Detection of α-synuclein in CSF by RT-QuIC in patients with isolated rapid-eye-movement sleep behaviour disorder: a longitudinal observational study

Alex Iranzo et al.

Summary: The study found that RT-QuIC can detect misfolded alpha-synuclein in the CSF of patients with IRBD with both high sensitivity and specificity, indicating that detection of alpha-synuclein may serve as an early marker for Parkinson's disease and dementia with Lewy bodies.

LANCET NEUROLOGY (2021)

Article Multidisciplinary Sciences

Patients with scans without evidence of dopaminergic deficit (SWEDD) do not have early Parkinson's disease: Analysis of the PPMI data

Jeong Won Lee et al.

Summary: Evaluation of SWEDD patients and PD patients revealed that SWEDD may not be early PD but rather a different disease entity based on clinical characteristics and SPECT imaging parameters.

PLOS ONE (2021)

Article Clinical Neurology

Alpha-Synuclein Oligomers and Neurofilament Light Chain Predict Phenoconversion of Pure Autonomic Failure

Wolfgang Singer et al.

Summary: NfL levels at baseline were only elevated in patients who later converted to MSA, perfectly distinguishing them from future PD and DLB converters as well as non-converters. ASyn-PMCA was positive in almost all cases, with marked differences in reaction kinetics seen in samples from patients who later developed MSA. This finding has important implications for prognostication and differentiation of MSA from other synucleinopathies before motor symptoms appear.

ANNALS OF NEUROLOGY (2021)

Article Clinical Neurology

Alpha-synuclein seeds in olfactory mucosa of patients with isolated REM sleep behaviour disorder

Ambra Stefani et al.

Summary: RT-QuIC detection of alpha-synuclein aggregates in olfactory mucosa showed a linkage between alpha-synuclein pathology and olfactory dysfunction in patients with isolated RBD and Parkinson's disease, indicating a potential for early detection and preventive therapies.

BRAIN (2021)

Article Clinical Neurology

RT-QuIC Detection of Pathological α-Synuclein in Skin Punches of Patients with Lewy Body Disease

Angela Mammana et al.

Summary: Skin alpha-synuclein real-time quaking-induced conversion assay can accurately distinguish patients with Lewy body disease. Furthermore, the two real-time quaking-induced conversion assay protocols showed similar diagnostic accuracy when analyzing both CSF and skin samples from patients.

MOVEMENT DISORDERS (2021)

Article Clinical Neurology

In Vivo Diagnosis of Synucleinopathies

Vincenzo Donadio et al.

Summary: The study demonstrates that both immunofluorescence and RT-QuIC have high diagnostic accuracy in distinguishing synucleinopathies from non-synucleinopathies, with immunofluorescence showing higher diagnostic accuracy and a good level of agreement with RT-QuIC in both skin and CSF.

NEUROLOGY (2021)

Article Neurosciences

Streamlined alpha-synuclein RT-QuIC assay for various biospecimens in Parkinson's disease and dementia with Lewy bodies

Connor Bargar et al.

Summary: The development of a streamlined RT-QuIC assay based on commercial reagents allows for uniform analysis of alpha Syn(D) in various biospecimens from PD and DLB cases, achieving high sensitivity and specificity in detecting disease-associated aggregates. The use of this assay can improve diagnostic applications and facilitate efficient utilization of scarce biospecimens for understanding disease pathogenesis and monitoring therapeutic efficacies.

ACTA NEUROPATHOLOGICA COMMUNICATIONS (2021)

Letter Clinical Neurology

Seed Amplification Assay to Diagnose Early Parkinson's and Predict Dopaminergic Deficit Progression

Luis Concha-Marambio et al.

MOVEMENT DISORDERS (2021)

Article Clinical Neurology

In Vivo Diagnosis of Synucleinopathies A Comparative Study of Skin Biopsy and RT-QuIC

Vincenzo Donadio et al.

Summary: This study demonstrates that both immunofluorescence and RT-QuIC show high diagnostic accuracy in distinguishing synucleinopathies from non-synucleinopathies, with immunofluorescence displaying better value and optimal reproducibility. Immunofluorescence exhibits good agreement with RT-QuIC in synucleinopathies in both skin and CSF.

NEUROLOGY (2021)

Article Clinical Neurology

Alpha-synuclein seeds in olfactory mucosa and cerebrospinal fluid of patients with dementia with Lewy bodies

Daniela Perra et al.

Summary: The study examined the diagnostic accuracy of alpha-synuclein real-time quaking-induced conversion assay in detecting alpha-synuclein in olfactory mucosa and CSF of patients with suspected dementia with Lewy bodies. The results suggest that this approach could optimize clinical diagnosis and potentially improve concordance with clinical diagnosis.

BRAIN COMMUNICATIONS (2021)

Article Multidisciplinary Sciences

Discriminating α-synuclein strains in Parkinson's disease and multiple system atrophy

Mohammad Shahnawaz et al.

NATURE (2020)

Article Multidisciplinary Sciences

Serum neurofilament light levels in normal aging and their association with morphologic brain changes

Michael Khalil et al.

NATURE COMMUNICATIONS (2020)

Article Clinical Neurology

Validation of Serum Neurofilament Light Chain as a Biomarker of Parkinson's Disease Progression

Brit Mollenhauer et al.

MOVEMENT DISORDERS (2020)

Article Clinical Neurology

α-synuclein RT-QuIC in cerebrospinal fluid of LRRK2-linked Parkinson's disease

Alicia Garrido et al.

ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY (2019)

Article Multidisciplinary Sciences

Ultrasonication-based rapid amplification of α-synuclein aggregates in cerebrospinal fluid

Keita Kakuda et al.

SCIENTIFIC REPORTS (2019)

Article Clinical Neurology

α-Synuclein RT-QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies

Matilde Bongianni et al.

ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY (2019)

Article Clinical Neurology

The REM Sleep Behavior Disorder Screening Questionnaire is not Valid in De Novo Parkinson's Disease

Claire Halsband et al.

MOVEMENT DISORDERS CLINICAL PRACTICE (2018)

Article Clinical Neurology

Neuropathological basis of nonmotor manifestations of Parkinson's disease

Charles H. Adler et al.

MOVEMENT DISORDERS (2016)

Article Clinical Neurology

Alpha-synuclein RT-QuIC in the CSF of patients with alpha-synucleinopathies

Graham Fairfoul et al.

ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY (2016)

Article Clinical Neurology

Patients With Scans Without Evidence of Dopaminergic Deficit: A Long-Term Follow-up Study

Amit Batla et al.

MOVEMENT DISORDERS (2014)

Article Clinical Neurology

Questionnaire-based diagnosis of REM sleep behavior disorder in Parkinson's disease

Lama M. Chahine et al.

MOVEMENT DISORDERS (2013)

Article Clinical Neurology

Role of DaTSCAN and clinical diagnosis in Parkinson disease

Raul de la Fuente-Fernandez

NEUROLOGY (2012)