Journal
JOURNAL OF CLINICAL MEDICINE
Volume 10, Issue 21, Pages -Publisher
MDPI
DOI: 10.3390/jcm10215197
Keywords
diagnosis; infectious disease; lifestyle-related disease; ultrasensitive ELISA; thio-NAD cycling
Categories
Funding
- Waseda Research Institute for Science and Engineering, Waseda University
- JST [AS3015096U, JPMJTM20LW]
- JSPS [20H04556]
- Grants-in-Aid for Scientific Research [20H04556] Funding Source: KAKEN
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ELISA is a useful tool for quantitative measurement of proteins, and enhancing its detection sensitivity to the ultrasensitive level can improve disease diagnosis. This review article discusses the definition of 'ultrasensitive', surveys current literature on modified ELISA methods with ultrasensitive detection, and introduces a newly devised system for ultrasensitive ELISA application.
An enzyme-linked immunosorbent assay (ELISA) can be used for quantitative measurement of proteins, and improving the detection sensitivity to the ultrasensitive level would facilitate the diagnosis of various diseases. In the present review article, we first define the term 'ultrasensitive'. We follow this with a survey and discussion of the current literature regarding modified ELISA methods with ultrasensitive detection and their application for diagnosis. Finally, we introduce our own newly devised system for ultrasensitive ELISA combined with thionicotinamide adenine dinucleotide cycling and its application for the diagnosis of infectious diseases and lifestyle-related diseases. The aim of the present article is to expand the application of ultrasensitive ELISAs in the medical and biological fields.
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