Journal
JOURNAL OF CLINICAL MEDICINE
Volume 10, Issue 22, Pages -Publisher
MDPI
DOI: 10.3390/jcm10225324
Keywords
apurinic/apyrimidinic endonuclease 1/redox factor-1; rheumatoid arthritis; DAS28; ELISA
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Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2014R1A6A1029617]
- Chungnam National University Hospital Research Fund
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The study demonstrates that the levels of APE1/Ref-1 are significantly elevated in the serum and synovial fluid of rheumatoid arthritis patients, and are positively correlated with disease activity.
There is growing evidence that apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) regulates inflammatory responses. Rheumatoid arthritis (RA) is an autoimmune disease, which is characterized with synovitis and joint destruction. Therefore, this study was planned to investigate the relationship between APE1/Ref-1 and RA. Serum and synovial fluid (SF) were collected from 46 patients with RA, 45 patients with osteoarthritis (OA), and 30 healthy control (HC) patients. The concentration of APE1/Ref-1 in serum or SF was measured using the sandwich enzyme-linked immunosorbent assay (ELISA). The disease activity in RA patients was measured using the 28-joint disease activity score (DAS28). The serum APE1/Ref-1 levels in RA patients were significantly increased compared to HC and OA patients (0.44 & PLUSMN; 0.39 ng/mL for RA group vs. 0.19 & PLUSMN; 0.14 ng/mL for HC group, p < 0.05 and vs. 0.19 & PLUSMN; 0.11 ng/mL for OA group, p < 0.05). Likewise, the APE1/Ref-1 levels of SF in RA patients were also significantly increased compared to OA patients (0.68 & PLUSMN; 0.30 ng/mL for RA group vs. 0.31 & PLUSMN; 0.12 ng/mL for OA group, p < 0.001). The APE1/Ref-1 concentration in SF of RA patients was positively correlated with DAS28. Thus, APE1/Ref-1 may reflect the joint inflammation and be associated with disease activity in RA.
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