Capturing an elusive but critical element: Natural protein enables actinium chemistry

Actinium-based therapies could revolutionize cancer medicine but remain tantalizing due to the difficulties in studying and limited knowledge of Ac chemistry. Current efforts focus on small synthetic chelators, limiting radioisotope complexation and purification efficiencies. Here, we demonstrate a straightforward strategy to purify medically relevant radiometals, actinium(III) and yttrium(III), and probe their chemistry, using the recently discovered protein, lanmodulin. The stoichiometry, solution behavior, and formation constant of the Ac-228(3+)-lanmodulin complex and its Y-90(3+)/Y-nat(3+)/La-nat(3+) a analogs were experimentally determined, representing the first actinium-protein and strongest actinide(III)-protein complex (sub-picomolar K-d) to be characterized. Lanmodulin's unparalleled properties enable the facile purification recovery of radiometals, even in the presence of >10(+10) equivalents of competing ions and at ultratrace levels: down to 2 femtograms Y-90(3+) and 40 attograms Ac-228(3+). The lanmodulin-based approach charts a new course to study elusive isotopes and develop versatile chelating platforms for medical radiometals, both for high-value separations and potential in vivo applications.

Automated summary

The study demonstrates a straightforward strategy using the protein lanmodulin to purify medically relevant radiometals, probing their chemistry and experimentally determining the properties of the Ac-228(3+)-lanmodulin complex and its analogs. Lanmodulin's unparalleled properties allow for easy purification and recovery of radiometals at ultratrace levels, opening up new possibilities for studying elusive isotopes and developing versatile chelating platforms for medical radiometals.