Journal
SCIENCE ADVANCES
Volume 7, Issue 47, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abk1151
Keywords
-
Categories
Funding
- CSIRO from the BMGF (USA) [OPP1076280]
- Gordon and Betty Moore Foundation [GBMF3068]
- Howard Hughes Medical Institute (HHMI)
- Rijk Zwaan Zaadteelten Zaadhandel B.V.
- East-West Seed International
- Ministry of Education (MoE), Government of India (GoI) [STARS/APR2019/BS/818/FS]
- Focus basic research in the Agriculture Nutrition Biotechnology Theme project from CSIR, Ministry of Science and Technology, GoI [MLP0120 Np.31-2(281)/208-19/budget]
- DBTRamalingaswami Re-entry Fellowship awarded by the Department of Biotechnology, GoI
- UGC-JRF PhD fellowship - University Grants Commission (UGC), GoI
- MoE-STARS project senior research fellowship
- Syngenta
- Bill and Melinda Gates Foundation [OPP1076280] Funding Source: Bill and Melinda Gates Foundation
Ask authors/readers for more resources
The study reveals that alterations in centromeric histone H3 can lead to selective removal of one parental chromosome set in hybrid zygotes, with the mechanism involving cooperative binding of CENH3 to chromatin. This epigenetic difference results in strong mating barriers and the production of haploids in hybrid crosses.
Wide crosses result in postzygotic elimination of one parental chromosome set, but the mechanisms that result in such differential fate are poorly understood. Here, we show that alterations of centromeric histone H3 (CENH3) lead to its selective removal from centromeres of mature Arabidopsis eggs and early zygotes, while wild-type CENH3 persists. In the hybrid zygotes and embryos, CENH3 and essential centromere proteins load preferentially on the CENH3-rich centromeres of the wild-type parent, while CENH3-depleted centromeres fail to reconstitute new CENH3-chromatin and the kinetochore and are frequently lost. Genome elimination is opposed by E3 ubiquitin ligase VIM1. We propose a model based on cooperative binding of CENH3 to chromatin to explain the differential CENH3 loading rates. Thus, parental CENH3 polymorphisms result in epigenetically distinct centromeres that instantiate a strong mating barrier and produce haploids.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available