Journal
SCIENCE ADVANCES
Volume 7, Issue 50, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abk0568
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Funding
- German research foundation (DFG) [SPP1879]
- Max Planck Society
- DFG-Cluster of Excellence Controlling Microbes to Fight Infections [EXC 2124]
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Cyanobacteria rely on c-di-AMP mediated SbtB regulation of glycogen metabolism to adapt to day-night metabolic changes. SbtB interacts specifically with c-di-AMP in light and darkness, affecting glycogen synthesis and nighttime survival of cyanobacteria.
Because of their photosynthesis-dependent lifestyle, cyanobacteria evolved sophisticated regulatory mechanisms to adapt to oscillating day-night metabolic changes. How they coordinate the metabolic switch between autotrophic and glycogen-catabolic metabolism in light and darkness is poorly understood. Recently, c-di-AMP has been implicated in diurnal regulation, but its mode of action remains elusive. To unravel the signaling functions of c-di-AMP in cyanobacteria, we isolated c-di-AMP receptor proteins. Thereby, the carbon-sensor protein SbtB was identified as a major c-di-AMP receptor, which we confirmed biochemically and by x-ray crystallography. In search for the c-di-AMP signaling function of SbtB, we found that both SbtB and c-di-AMP cyclase-deficient mutants showed reduced diurnal growth and that c-di-AMP-bound SbtB interacts specifically with the glycogen-branching enzyme GlgB. Accordingly, both mutants displayed impaired glycogen synthesis during the day and impaired nighttime survival. Thus, the pivotal role of c-di-AMP in day-night acclimation can be attributed to SbtB-mediated regulation of glycogen metabolism.
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