Journal
ACS SENSORS
Volume 6, Issue 11, Pages 4001-4008Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.1c01371
Keywords
fluorescence; saccharide sensing; sensor array; dual chromophore sensor systems; urine analysis
Funding
- Bowling Green State University (Building Strength Award) [33000211]
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The developed dual chromophore sensors in this study can accurately monitor glucose levels in urine, successfully classify nine different sugars, and quantitatively determine important carbohydrates without the need for sample pretreatment.
Accurate monitoring of sugar levels is essential for many fields from food industry to human health. Here, we developed FRET-based dual chromophore sensors for saccharides that form oxazolidine boronate and may be employed as a noninvasive method for monitoring of sugar levels in biological fluids, namely, urine. The saccharide-binding properties of the sensors were studied using fluorescence spectroscopy and utilized in the determination of saccharides in a high-throughput manner. Here, two fluorescent sensors were successful in the classification of nine different monosaccharides and disaccharides with 100% correct classification. Furthermore, the dual chromophore self-assembled sensors were successfully utilized for the quantitative determination of important carbohydrates such as glucose in the presence of competitive saccharides (fructose) and in complex media (urine) without sample pretreatment. The present fluorescent sensors allow for quantification of glucose in a concentration range of 0-60 mM, which matches the concentration range of frequently used urinalysis test strips.
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