Square Wave Voltammetric Determination of Rasagiline Mesylate on Hanging Mercury Drop Electrode and its application in Dosage Form and Biological Fluids

A new square wave voltammetric (SWV) method has been developed and validated for the determination of Rasagiline mesylate in bulk, dosage form and human plasma due to a cathodic behaviour of its acetylene group at Hanging Mercury Drop Electrode (HMDE). Many facts such as different supporting electrolytes, scan rate, pulse amplitude, accumulation time and accumulation potential were studied. A good linearity was obtained over a concentration range of (2.8 x 10(-8) to1.4 x 10(-7)mol L-1) with 99.78% as mean recovery and 1.09%, as the relative standard deviation and limits of detection and quantification were found to be (2.7 x 10(-9) and 9.24 x 10(-9)mol L-1), respectively. The accuracy and precision of the method were within acceptable limits. The method was applied for determining the active ingredients in its tablets with % recoveries +/- relative standard deviation of 95.46 +/- 3.7 6 and in spiked human plasma with 94.3 +/- 2.8 9%, respectively.