Factorial design-assisted spectroscopic determination of oxybutynin hydrochloride

In this study, we have developed two facile spectroscopic methods for quantifying oxybutynin (OBT) hydrochloride in its pure form and tablets using design of experiments (DOEs). The spectroscopic methods depended on the ion-pair complex formation between the tertiary amino group in the drug and eosin in 0.2 M acetate buffer of pH 4. Method I involves spectrophotometric measurement of the absorbance of the developed complex at 550 nm and showed linearity through 1.0-10.0 mu g ml(-1). Method II involves spectrofluorometric measurement of the quenching influence of OBT on the native fluorescence of eosin (lambda excitation/lambda emission of 304/548 nm) and showed linearity through 1.0-6.0 mu g ml(-1). Critical parameters were identified through preliminary trials and optimized using the DOE. Additionally, the quenching mechanism was investigated and the pathway of the reaction was postulated. The fluorescence quenching constant and thermodynamic parameters were explored using the Stern-Volmer plot and Van't Hoff graph, respectively. Assessments conducted via analytical ecoscale revealed the 'excellent-greenness' of the methodology. The two methods have the potentials of being green and fast compared with other reported methods.

Automated summary

Two spectroscopic methods were developed for quantifying oxybutynin hydrochloride, dependent on ion-pair complex formation between the drug's tertiary amino group and eosin in acetate buffer. The methods showed linearity in a specific concentration range, and greenness and speed were highlighted as potentials compared to other reported methods. Further investigation into quenching mechanism and reaction pathway was conducted to provide insights into the methodology.