Journal
FRONTIERS IN GENETICS
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fgene.2021.803683
Keywords
ring chromosome 21; trisomy 21; RNA-Seq; induced pluripotent stem cells; neuroepithelial stem cells; microarray; genomic deletion; chromosomal abnormalities
Categories
Funding
- Swedish Research Council [2019-01498, 2020-01947]
- Stockholm Regional Council
- Strategic Research Program in Neuroscience at Karolinska Institutet (StratNeuro)
- Swedish Brain Foundation [FO 2020-0351, FO 2019-0246]
- Swedish Foundation for Strategic Research [IB13-0074]
- Hjaernfonden [FO 2020-0171]
- [sens2017106]
- [sens2017130]
- [2017-02936]
- [2019-02078]
- Vinnova [2019-01498] Funding Source: Vinnova
- Swedish Research Council [2019-01498, 2020-01947] Funding Source: Swedish Research Council
- Forte [2019-01498] Funding Source: Forte
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Using induced pluripotent stem cells (iPSCs) from patients, researchers investigated the effects of partial monosomy on neural cells. RNA-Seq analysis revealed downregulation of multiple genes within the deleted region and global transcriptional dysregulation. A comparison with trisomy 21 cell lines showed opposite expression changes for genes on chromosome 21 and non-chromosome 21 genes.
Induced pluripotent stem cells (iPSCs) from patients are an attractive disease model to study tissues with poor accessibility such as the brain. Using this approach, we and others have shown that trisomy 21 results in genome-wide transcriptional dysregulations. The effects of loss of genes on chromosome 21 is much less characterized. Here, we use patient-derived neural cells from an individual with neurodevelopmental delay and a ring chromosome 21 with two deletions spanning 3.8 Mb at the terminal end of 21q22.3, containing 60 protein-coding genes. To investigate the molecular perturbations of the partial monosomy on neural cells, we established patient-derived iPSCs from fibroblasts retaining the ring chromosome 21, and we then induced iPSCs into neuroepithelial stem cells. RNA-Seq analysis of NESCs with the ring chromosome revealed downregulation of 18 genes within the deleted region together with global transcriptomic dysregulations when compared to euploid NESCs. Since the deletions on chromosome 21 represent a genetic contrary to trisomy of the corresponding region, we further compared the dysregulated transcriptomic profile in with that of two NESC lines with trisomy 21. The analysis revealed opposed expression changes for 23 genes on chromosome 21 as well as 149 non-chromosome 21 genes. Taken together, our results bring insights into the effects on the global and chromosome 21 specific gene expression from a partial monosomy of chromosome 21qter during early neuronal differentiation.
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