Journal
FRONTIERS IN GENETICS
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fgene.2021.794157
Keywords
microRNA; goat kid; liver; development; ruminant
Categories
Funding
- Shandong Provincial Modern Agriculture Industry Technology System [SDAIT-10]
- Funds of the Shandong Double Tops Program [SYL2017YSTD12]
- project of improved agricultural varieties in Shandong Province [2019LZGC012, 2021LZGC010]
Ask authors/readers for more resources
This study sequenced miRNAs in the liver tissues of goat kids and investigated their regulation roles in early liver development. The results showed that differential expression miRNAs played important roles in liver development, lipid metabolism, and toxin-related metabolism. Furthermore, miRNA-mRNA regulation network analysis revealed 9 miRNAs with significant regulatory roles.
The liver is the largest digestive gland in goats with an important role in early metabolic function development. MicroRNAs (miRNA) are crucial for regulating the development and metabolism in the goat liver. In the study, we sequenced the miRNAs in the liver tissues of the goat kid to further research their regulation roles in early liver development. The liver tissues were procured at 5-time points from the Laiwu black goats of 1 day (D1), 2 weeks (W2), 4 weeks (W4), 8 weeks (W8), and 12 weeks (W12) after birth, respectively with five goats per time point, for a total of 25 goats. Our study identified 214 differential expression miRNAs, and the expression patterns of 15 randomly selected miRNAs were examined among all five age groups. The Gene ontology annotation results showed that differential expression miRNA (DE miRNA) target genes were significantly enriched in the fatty acid synthase activity, toxin metabolic process, cell surface, and antibiotic metabolic process. The KEGG analysis result was significantly enriched in steroid hormone synthesis and retinol metabolism pathways. Further miRNA-mRNA regulation network analysis reveals 9 differently expressed miRNA with important regulation roles. Overall, the DE miRNAs were mainly involved in liver development, lipid metabolism, toxin related metabolism-related biological process, and pathways. Our results provide new information about the molecular mechanisms and pathways in the goat kid liver development.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available