Analysis of N6-Methyladenosine Methylome in Adenocarcinoma of Esophagogastric Junction

Background: From previous studies, we found that there are more than 100 types of RNA modifications in RNA molecules. m(6)A methylation is the most common. The incidence rate of adenocarcinoma of the esophagogastric junction (AEG) at home and abroad has increased faster than that of stomach cancer at other sites in recent years. Here, we systematically analyze the modification pattern of m(6)A mRNA in adenocarcinoma at the esophagogastric junction.Methods: m(6)A sequencing, RNA sequencing, and bioinformatics analysis were used to describe the m(6)A modification pattern in adenocarcinoma and normal tissues at the esophagogastric junction.Results: In AEG samples, a total of 4,775 new m(6)A peaks appeared, and 3,054 peaks disappeared. The unique m6A-related genes in AEG are related to cancer-related pathways. There are hypermethylated or hypomethylated m(6)A peaks in AEG in differentially expressed mRNA transcripts.Conclusion: This study preliminarily constructed the first m(6)A full transcriptome map of human AEG. This has a guiding role in revealing the mechanism of m(6)A-mediated gene expression regulation.

Automated summary

This study systematically analyzed the m(6)A mRNA modification pattern in adenocarcinoma at the esophagogastric junction, identifying numerous new m(6)A peaks and their relationship with cancer-related pathways in AEG samples. The differential expression of mRNA transcripts in AEG showed hypermethylated or hypomethylated m(6)A peaks.