Journal
TRANSLATIONAL CANCER RESEARCH
Volume 10, Issue 10, Pages 4478-4488Publisher
AME PUBLISHING COMPANY
DOI: 10.21037/tcr-21-1940
Keywords
Long noncoding RNA (IncRNA); RNA-binding protein; maintenance complex component 3 associated protein antisense RNA 1 (MCM3AP-AS1); ZFP36 ring finger protein (ZFP36); cell cycle
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The study revealed that lncRNA MCM3AP-AS1 is significantly upregulated in breast cancer and correlates with clinicopathological characteristics. Downregulation of MCM3AP-AS1 inhibits proliferation, apoptosis, migration, and invasion of breast cancer cells. Further analysis shows that MCM3AP-AS1 interacts with ZFP36 to regulate the levels of several proteins.
Background: Previous studies suggest that long noncoding RNA (IncRNA) maintenance complex component 3 associated protein (MCM3AP) antisense RNA 1 (MCM3AP-AS I) has a wide range of functions in several cancers. However, its expression and functions in breast cancer are unclear. Methods: Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of MCM3AP-AS1. MTT, colony formation and anchorage-independent growth assay were used to examine the effect of MCM3AP-AS1 on growth of breast cancer cells. TUNE!. and flow cytometry assay were applied to investigate the role of MCM3AP-AS1 on cell apoptosis. Wound heading and transwell matric penetration assay were used to detect the role of MCM3AP-AS1 on cell motility. RNA pull-down and RIP assay were used to examine the interaction of MCM3AP-AS 1 and ZFP36. Results: We found that IncRNA MCM3AP-AS I was significantly upregulated in breast cancer, which correlated with patients' clinicopathological characteristics. Downregulation of MCNI3AP-AS1 substantially inhibited the proliferation, apoptosis, migration, and invasion of breast cancer cells. Further analysis clarified that MCM3AP-AS1 binds with the RNA-binding protein ZFP36 ring finger protein (ZFP36) to regulate the levels of cyclin D1 (CCND1), c-myc (MYC), and matrix metalloproteinase 1 (MMP1). Conclusions: Our findings show IncRNA MCM3AP-AS1 might be a prognostic Factor and a promising therapeutic target for breast cancer therapy.
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