Journal
REDOX BIOLOGY
Volume 47, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.redox.2021.102140
Keywords
Diabetes; Heart; Cardiomyocytes; EPR; Thioredoxin; Insulin
Categories
Funding
- National Institutes of Health [R01HL125625, P30AG050911, P20GM103447, R24GM137786]
- Oklahoma Center for the Advancement of Science [HR17-094]
- National Science Foundation [1849507]
- Direct For Education and Human Resources
- Division Of Graduate Education [1849507] Funding Source: National Science Foundation
Ask authors/readers for more resources
The study reveals that insulin can mitigate cardiac oxidative stress in diabetes by regulating endogenous antioxidant activity, with thioredoxin 1 having the ability to directly scavenge superoxide radicals.
Diabetic cardiomyopathy is associated with an increase in oxidative stress. However, antioxidant therapy has shown a limited capacity to mitigate disease pathology. The molecular mechanisms responsible for the modulation of reactive oxygen species (ROS) production and clearance must be better defined. The objective of this study was to determine how insulin affects superoxide radical (O-2(center dot-)) levels. O-2(center dot-) production was evaluated in adult cardiomyocytes isolated from control and Akita (type 1 diabetic) mice by spin-trapping electron paramagnetic resonance spectroscopy. We found that the basal rates of O-2(center dot-) production were comparable in control and Akita cardiomyocytes. However, culturing cardiomyocytes without insulin resulted in a significant increase in O-2(center dot-) production only in the Akita group. In contrast, O-2(center dot-) production was unaffected by high glucose and/or fatty acid supplementation. The increase in O-2(center dot-) was due in part to a decrease in superoxide dismutase (SOD) activity. The PI3K inhibitor, LY294002, decreased Akita SOD activity when insulin was present, indicating that the modulation of antioxidant activity is through insulin signaling. The effect of insulin on mitochondrial O-2(center dot)-production was evaluated in Akita mice that underwent a 1-week treatment of insulin. Mitochondria isolated from insulin-treated Akita mice produced less O-2(center dot-) than vehicle-treated diabetic mice. Quantitative proteomics was performed on whole heart homogenates to determine how insulin affects antioxidant protein expression. Of 29 antioxidant enzymes quantified, thioredoxin 1 was the only one that was significantly enhanced by insulin treatment. In vitro analysis of thioredoxin 1 revealed a previously undescribed capacity of the enzyme to directly scavenge O-2(center dot-). These findings demonstrate that insulin has a role in mitigating cardiac oxidative stress in diabetes via regulation of endogenous antioxidant activity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available