Journal
FRONTIERS IN IMMUNOLOGY
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2021.736479
Keywords
Sinomenine; airway remodeling; asthma; EMT; TGF-beta 1; Smad3 expression
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Funding
- Henan Province Scientific and Technological Project [212102310344,202102310472]
- Key scientific research projects of colleges and universities in Henan Province [20A360009]
- Henan University of Traditional Chinese Medicine Doctoral Research Fund [RSBSJJ2018-12]
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Sin suppresses epithelial-mesenchymal transition (EMT) by inhibiting IL-4 and downregulating TGF-beta 1 and Smad3 expression, thereby alleviating airway remodeling and inflammation.
Airway remodeling is associated with dysregulation of epithelial-mesenchymal transition (EMT) in patients with asthma. Sinomenine (Sin) is an effective, biologically active alkaloid that has been reported to suppress airway remodeling in mice with asthma. However, the molecular mechanisms behind this effect remain unclear. We aimed to explore the potential relationship between Sin and EMT in respiratory epithelial cells in vitro and in vivo. First, 16HBE cells were exposed to 100 mu g/mL LPS and treated with 200 mu g/mL Sin. Cell proliferation, migration, and wound healing assays were performed to evaluate EMT, and EMT-related markers were detected using Western blotting. Mice with OVA-induced asthma were administered 35 mg/kg or 75 mg/kg Sin. Airway inflammation and remodeling detection experiments were performed, and EMT-related factors and proteins in the TGF-beta 1 pathway were detected using IHC and Western blotting. We found that Sin suppressed cell migration but not proliferation in LPS-exposed 16HBE cells. Sin also inhibited MMP7, MMP9, and vimentin expression in 16HBE cells and respiratory epithelial cells from mice with asthma. Furthermore, it decreased OVA-specific IgE and IL-4 levels in serum, relieved airway remodeling, attenuated subepithelial collagen deposition, and downregulating TGF-beta 1and Smad3 expression in mice with asthma. Our results suggest that Sin suppresses EMT by inhibiting IL-4 and downregulating TGF-beta 1 and Smad3 expression.
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