Transcriptome Profiling of Porcine Naive, Intermediate and Terminally Differentiated CD8+ T Cells

The pig has the potential to become a leading research model for human diseases, pharmacological and transplantation studies. Since there are many similarities between humans and pigs, especially concerning anatomy, physiology and metabolism, there is necessity for a better understanding of the porcine immune system. In adaptive immunity, cytotoxic T lymphocytes (CTLs) are essential for host defense. However, most data on CTLs come from studies in mice, non-human primates and humans, while detailed information about porcine CD8(+) CTLs is still sparse. Aim of this study was to analyze transcriptomes of three subsets of porcine CD8 beta(+) T-cell subsets by using next-generation sequencing technology. Specifically, we described transcriptional profiles of subsets defined by their CD11a/CD27 expression pattern, postulated as naive (CD8 beta(+)CD27(+)CD11a(low)), intermediate differentiated (CD8 beta(+)CD27(dim)CD11a(+)), and terminally differentiated cells (CD8 beta(+)CD27(-)CD11a(high)). Cells were analyzed in ex vivo condition as well as upon in vitro stimulation with concanavalin A (ConA) and PMA/ionomycin. Our analyses show that the highest number of differentially expressed genes was identified between naive and terminally differentiated CD8(+) T-cell subsets, underlining their difference in gene expression signature and respective differentiation stages. Moreover, genes related to early (IL7-R, CCR7, SELL, TCF7, LEF1, BACH2, SATB1, ZEB1 and BCL2) and late (KLRG1, TBX21, PRDM1, CX3CR1, ZEB2, ZNF683, BATF, EZH2 and ID2) stages of CD8(+) T-cell differentiation were highly expressed in the naive and terminally differentiated CD8(+) T-cell subsets, respectively. Intermediate differentiated CD8(+) T-cell subsets shared a more comparable gene expression profile associated with later stages of T-cell differentiation. Genes associated with cytolytic activity (GNLY, PRF1, GZMB, FASL, IFNG and TNF) were highly expressed in terminally and intermediate differentiated CD8(+) T-cell subsets, while naive CD8(+) T cells lacked expression even after in vitro stimulation. Overall, PMA/ionomycin stimulation induced much stronger upregulation of genes compared to stimulation with ConA. Taken together, we provided comprehensive results showing transcriptional profiles of three differentiation stages of porcine CD8(+) T-cell subsets. In addition, our study provides a powerful toolbox for the identification of candidate markers to characterize porcine immune cell subsets in more detail.

Automated summary

Pigs have the potential to be a valuable research model for studying human diseases, pharmacology, and transplantation. This study aimed to better understand the porcine immune system by analyzing the transcriptomes of different subsets of porcine CD8(+) T cells. The results showed distinct gene expression patterns in different subsets, providing valuable information for further research on porcine immune cell subsets.