Journal
FRONTIERS IN IMMUNOLOGY
Volume 13, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2022.750660
Keywords
cytotoxicity; granzyme B; neuroinflammation; neurodegeneration; MS treatment
Categories
Funding
- Sao Paulo Research Foundation (FAPESP) [2014/26431-0, 2015/22052-8, 2017/21363-5, 2019/06372-3, 2019/16116-4]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brasil (CAPES) [001 (2015/22052-8)]
Ask authors/readers for more resources
This study found that CD19(+) B cells in the peripheral blood of multiple sclerosis (MS) patients may express serine-protease granzyme-B (GzmB), resembling cytotoxic CD8(+) T lymphocytes. This finding may provide important clues for investigating the pathogenesis of MS and for the development of new therapeutic interventions.
BackgroundEmerging evidence of antibody-independent functions, as well as the clinical efficacy of anti-CD20 depleting therapies, helped to reassess the contribution of B cells during multiple sclerosis (MS) pathogenesis. ObjectiveTo investigate whether CD19(+) B cells may share expression of the serine-protease granzyme-B (GzmB), resembling classical cytotoxic CD8(+) T lymphocytes, in the peripheral blood from relapsing-remitting MS (RRMS) patients. MethodsIn this study, 104 RRMS patients during different treatments and 58 healthy donors were included. CD8, CD19, Runx3, and GzmB expression was assessed by flow cytometry analyses. ResultsRRMS patients during fingolimod (FTY) and natalizumab (NTZ) treatment showed increased percentage of circulating CD8(+)GzmB(+) T lymphocytes when compared to healthy volunteers. An increase in circulating CD19(+)GzmB(+) B cells was observed in RRMS patients during FTY and NTZ therapies when compared to glatiramer (GA), untreated RRMS patients, and healthy donors but not when compared to interferon-beta (IFN). Moreover, regarding Runx3, the transcriptional factor classically associated with cytotoxicity in CD8(+) T lymphocytes, the expression of GzmB was significantly higher in CD19(+)Runx3(+)-expressing B cells when compared to CD19(+)Runx3(-) counterparts in RRMS patients. ConclusionsCD19(+) B cells may exhibit cytotoxic behavior resembling CD8(+) T lymphocytes in MS patients during different treatments. In the future, monitoring cytotoxic subsets might become an accessible marker for investigating MS pathophysiology and even for the development of new therapeutic interventions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available