Genetic and viral approaches to record or manipulate neurons in insects

The development of genetically encoded tools to record and manipulate neurons in vivo has greatly increased our understanding of how neuronal activity affects behavior. Recent advances enable the use of these tools in species not typically considered genetically tractable. This progress is revolutionizing neuroscience in general, and insect neuroethology in particular. Here we cover the latest innovations and some of their applications in phylogenetically diverse insect species. We discuss the importance and implications of these approaches for both basic and translational research. We focus on genetically encoded and virally encoded tools used for calcium imaging, optogenetics, and synaptic silencing. Finally, we discuss potential future developments of universally applicable, modular, and user-friendly genetic toolkits for neuroethological studies of insect behavior.

Automated summary

The development of genetically encoded tools for recording and manipulating neurons has greatly enhanced our understanding of how neuronal activity influences behavior, especially in the realm of insect neuroethology. These tools have significant implications for both basic and translational research, with potential for future development of universally applicable, modular, and user-friendly genetic toolkits.