Journal
BIOENGINEERED
Volume 13, Issue 1, Pages 593-602Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/21655979.2021.2012405
Keywords
MiR-146; NF KB signaling pathway; rat; cardiopulmonary bypass; renal injury; protective effect
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The mechanism of renal injury after cardiopulmonary bypass and the protective effect of microRNA-146 through mediating NF-κB signaling pathway were investigated in this study. The results showed that the overexpression of miR-146 could inhibit inflammatory factors, cell apoptosis, and oxidative stress, thereby promoting the repair of renal injury.
The mechanism of renal injury after cardiopulmonary bypass is not clear, and the protective effect of microRNA-146 through mediating NF KB signaling pathway needs to be verified. The study intends to establish a rat model of cardiopulmonary bypass (CPB). MiR-146 is silenced or overexpressed by lentivirus transfection. It is divided into miR-146 inhibitors group (inhibitors), miR-146 mimics group (mimics) and sham group. It is found that the contents of Cr, bun and MDA in blood = , serum IL-1, IL-6 and TNF in mimics group are higher than those in the other two groups- alpha Content, apoptosis rate, ICAM-1, TNF- alpha, NF- kappa B mRNA and NF- kappa B protein decreased significantly (P < 0.05), while the content of SOD in kidney increased significantly (P < 0.05). In the inhibitors group, the above indicators showed the opposite results. Double luciferase assay showed that NF-kB was the target gene of miR-146. It can be seen that the expression of miR-146 inhibits inflammatory factors, apoptosis, oxidative stress and NF- kappa the activation of B pathway promotes the repair of renal injury in CPB rats.
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