Enamel Erosion Reduction through Coupled Sodium Fluoride and Laser Treatments before Exposition in an Acid Environment: An In Vitro Randomized Control SEM Morphometric Analysis

(1) Background: Erosive lesions of dental enamel are steadily increasing owing to both the availability of exogenous acid and the production of endogenous acid. The aim of this study was to investigate the erosion-inhibiting potential of a diode laser irradiation and topical application of fluoride used alone or in combination on the enamel surface of extracted teeth before exposure to an acidic solution. (2) Methods: The four axial enamel surfaces of 40 healthy molars were used for four study groups: (A) no treatment; (B) application of fluoride gel for 120 s; (O) a diode laser application for 120 s; and (X) a combined laser/fluoride for 120 s. Each enamel surface was examined by SEM (scanning electron microscopy). (3) Results: At 700x magnification, it was possible to detect the enamel prisms of the test area of groups A, B, and O, while no structures such as enamel prisms were highlighted for group X because they were covered by an amorphous layer. The mean number of prisms x 1000 mu m(2) was 7.2 units with an SD of 0.72 for group A, 8 units with an SD of 0.96 for group B, and 4.8 units with a SD of 0.4 for group O. Student's t-test showed no significant difference between group A and B with a p = 0.054. Group O showed a significant reduction of prims x1000 mu m(2) compared with group A (p = 0.0027) and group B (p = 0.0009). Student's t-test showed no significant difference between groups A and B with a p = 0.054. Group O showed a significant reduction of prims density with respect to group A (p = 0.0027) and group B (p = 0.0009). (4) Conclusions: This amorphous layer might be correlated with the effect of laser on enamel, which reduces both water and carbonate ion; increases the crystallinity of hydroxyapatite, and improves the mechanical properties of enamel; which is responsible for greater protection expressed by the enamel of group X against acid attacks.

Automated summary

This study investigated the protective effect of diode laser irradiation and topical application of fluoride on the erosion of dental enamel. The results showed that laser irradiation and fluoride application could reduce the number and density of enamel prisms, thereby improving the protection against acid erosion.