Journal
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
Volume 78, Issue -, Pages 14-29Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S2059798321011621
Keywords
time-resolved crystallography; serial crystallography; SSX; structural enzymology; mechanistic structural biology
Funding
- Deutsche Forschungsgemeinschaft (DFG) [458246365, 451079909]
- Joachim Herz Stiftung add-on fellowship
- Joachim Herz Stiftung via the Biomedical Physics of Infection Consortium
- Academy of Medical Sciences Springboard [SBF006\1044]
- Cluster of Excellence 'The Hamburg Centre for Ultrafast Imaging -Structure, Dynamics and Control of Matter at the Atomic Scale' of the Deutsche Forschungsgemeinschaft (DFG) [EXC1074]
- Cluster of Excellence 'CUI: Advanced Imaging of Matter' of the Deutsche Forschungsgemeinschaft (DFG) [EXC 2056, 390715994]
- BMBF ('Verbundforschung') [05K16GU1, 05K19GU1]
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With advancements in X-ray sources, equipment, and data-analysis tools, time-resolved crystallographic experiments are becoming more accessible to a wider user base. This article highlights the key factors that need to be considered when planning and conducting a time-resolved structural study, with a focus on synchrotron-based experiments.
With recent developments in X-ray sources, instrumentation and data-analysis tools, time-resolved crystallographic experiments, which were originally the preserve of a few expert groups, are becoming simpler and can be carried out at more radiation sources, and are thus increasingly accessible to a growing user base. However, these experiments are just that: discrete experiments, not just 'data collections'. As such, careful planning and consideration of potential pitfalls is required to enable a successful experiment. Here, some of the key factors that should be considered during the planning and execution of a time-resolved structural study are outlined, with a particular focus on synchrotron-based experiments.
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