Journal
ACS INFECTIOUS DISEASES
Volume 8, Issue 3, Pages 657-664Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsinfecdis.2c00011
Keywords
chemoenzymatic labeling; Neu5Aca(2-3)Gal epitope; Streptococcus agalactiae; Campylobacter jejuni
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Funding
- Shandong Natural Science Foundation [ZR2020QB157]
- Incubation Project of National Natural Science Foundation of China of Jining Medical University [700103164001]
- PhD Research Foundation of Affiliated Hospital of Jining Medical University [2020-BS-004]
- PhD Research Foundation of Jining Medical University [600874001]
- National Natural Science Foundation of China [32070921, 31770997]
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A rapid and sensitive chemoenzymatic method has been developed for detecting a specific structure on the surface of pathogens. This method shows potential for diagnosis and therapeutics of pathogenic infections.
The N-acetylneuraminic acid-alpha(2-3)-galactose epitope is often located at the nonreducing terminal ends of glycans on the envelopes of many pathogens, and it is believed that this structure mimics a host's oligosaccharide so as to circumvent and/or counteract the host's immune responses. A chemoenzymatic method for the rapid and sensitive detection of N-acetylneuraminic acid-alpha(2-3)-galactose has been built, so we planned to examine whether the chemoenzymatic method could be applied on the detection of N-acetylneuraminic acid-alpha(2-3)-galactose on pathogens. Our results revealed that the chemoenzymatic method was rapid and sensitive for labeling live or dead Gram- positive Streptococcus agalactiae A909 and Gram-negative Campylobacter jejuni MK104 with N-acetylneuraminic acid-alpha(2-3)galactose. This study suggested that the chemoenzymatic method was a new strategy for labeling pathogens and had potential for the diagnosis of or therapeutics for pathogenic infection.
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