Estimating the differences in critical thermal maximum and metabolic rate of Helicoverpa punctigera (Wallengren) (Lepidoptera: Noctuidae) across life stages

Temperature is a crucial driver of insect activity and physiological processes throughout their life-history, and heat stress may impact life stages (larvae, pupae and adult) in different ways. Using thermolimit respirometry, we assessed the critical thermal maxima (CTmax-temperature at which an organism loses neuromuscular control), CO2 emission rate (VCO2) and Q10 (a measure of VCO2 temperature sensitivity) of three different life stages of Helicoverpa punctigera (Wallengren) by increasing their temperature exposure from 25 degrees C to 55 degrees C at a rate of 0.25 degrees C min(-1). We found that the CTmax of larvae (49.1 degrees C +/- 0.3 degrees C) was higher than pupae (47. 4 degrees C +/- 0.2 degrees C) and adults (46.9 degrees C +/- 0.2 degrees C). The mean mass-specific CO2 emission rate (ml VCO2 h(-1)) of larvae (0.26 +/- 0.03 ml VCO2 h(-1)) was also higher than adults (0.24 +/- 0.04 ml VCO2 h(-1)) and pupae (0.06 +/- 0.02 ml VCO2 h(-1)). The Q10: 25-35 degrees C for adults (2.01 +/- 0.22) was significantly higher compared to larvae (1.40 +/- 0.06) and Q10: 35-45 degrees C for adults (3.42 +/- 0.24) was significant l y higher compared to larvae (1.95 +/- 0.08) and pupae (1.42 +/- 0.98) respectively. We have established the upper thermal tolerance of H. punctigera, which will lead to a better understanding of the thermal physiology of this species bot h in its na t i v e range, and as a pest species in agricultural systems.

Automated summary

The study on the thermal sensitivity of different life stages of Helicoverpa punctigera revealed that larvae had the highest critical thermal limit and CO2 emission rate, while the Q10 value of adults significantly increased with the rise in temperature.