Journal
NANOMATERIALS
Volume 11, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/nano11112775
Keywords
gold nanoparticles; siRNA; noncovalent adsorption; lipid enveloping; multilayer nanoconstructs for siRNA preparation and purification
Categories
Funding
- Russian Science Foundation [19-15-00217]
- Russian State Funded Project [121031300042-1]
- Russian Science Foundation [19-15-00217] Funding Source: Russian Science Foundation
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The study aimed to improve the quality of preparations of gold nanoparticle-based multilayer nanoconstructs (MLNCs) by finding optimal conditions for their fabrication, verifying all steps and purification processes, and ultimately optimizing the fabrication of target MLNCs bearing small interfering RNA.
There is an urgent need to develop systems for nucleic acid delivery, especially for the creation of effective therapeutics against various diseases. We have previously shown the feasibility of efficient delivery of small interfering RNA by means of gold nanoparticle-based multilayer nanoconstructs (MLNCs) for suppressing reporter protein synthesis. The present work is aimed at improving the quality of preparations of desired MLNCs, and for this purpose, optimal conditions for their multistep fabrication were found. All steps of this process and MLNC purification were verified using dynamic light scattering, transmission electron microscopy, and UV-Vis spectroscopy. Factors influencing the efficiency of nanocomposite assembly, colloidal stability, and purification quality were identified. These data made it possible to optimize the fabrication of target MLNCs bearing small interfering RNA and to substantially improve end product quality via an increase in its homogeneity and a decrease in the amount of incomplete nanoconstructs. We believe that the proposed approaches and methods will be useful for researchers working with lipid nanoconstructs.
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